《植物生理学报》 2012, 48(10): 993-996

领春木体细胞胚胎发生及植株再生

冉佳鑫, 王玉宇, 宋丹, 陈发菊*

三峡大学生物技术研究中心, 湖北宜昌443002

通信作者：陈发菊；E-mail: chenfj616@163.com；Tel: 0717-6397188

摘　要：

以领春木(Euptelea pleiospermum Hook. f. et Thoms.)种子幼胚为试验材料, 对领春木体细胞胚胎发生进行了研究。结 果表明: 在附加1.0 mg•L-1 2,4-D+0.5 mg•L-1 6-BA+3%蔗糖+0.8%琼脂的MS培养基上可诱导出愈伤组织。愈伤组织在附加0.5 mg•L-1 NAA+0.5 mg•L-1 6-BA的培养基上可形成体细胞胚; 体胚在附加0.5 mg•L-1 6-BA+0.05 mg•L-1 NAA+0.1% PVP的1/2MS培养基上能大量增殖; 将成熟体胚转移到不添加任何植物生长调节剂的MS培养基上, 培养60 d, 形成正常植株。

关键词：领春木; 组织培养; 体细胞胚胎发生

收稿：2012-07-19   修定：2012-08-07

资助：国家自然科学基金(30670202)。

Somatic Embryogenesis and Plant Regeneration of Euptelea pleiospermum Hook. f. et Thoms.

RAN Jia-Xin, WANG Yu-Yu, SONG Dan, CHEN Fa-Ju*

Biotechnology Research Center, China Three Gorges University, Yichang, Hubei 443002, China

Corresponding author: CHEN Fa-Ju; E-mail: chenfj616@163.com; Tel: 0717-6397188

Abstract:

Study on somatic embryogenesis from immature embryos in rare endangered plant Euptelea pleiospermum Hook. f. et Thoms. was achieved. The results showed that the best medium to induce the callus was MS+1.0 mg·L^-1 2,4-D+0.5 mg·L^-1 6-BA+3% sucrose +0.8% agar. The callus could transform to somatic embryo on the medium with 0.5 mg·L^-1 NAA+0.5 mg·L^-1 6-BA. A great quantity of somatic embryos could proliferate on 1/2MS+0.5 mg·L^-1 6-BA+0.05 mg·L^-1 NAA+0.1% PVP medium. The somatic embryos could develop to seedlings after 60 days on the medium of MS without plant growth regulator.

Key words: Euptelea pleiospermum Hook. f. et Thoms.; tissue culture; somatic embryogenesis